General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CHRM3 Total Internalization Assay, 2pl Kit. (93-0775E3CP5M)
The PathHunter CHRM3 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM3 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CHRM3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM3 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TGFBR1/TGFBR2 Dimerization Assay, 2pl Kit. (93-0889E3CP5M)
The PathHunter TGFBR1/TGFBR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TGF-β1) induced dimerization of TGFBR1/TGFBR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside TGFBR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/TGFBR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rhTrkA-rhP75 Functional Assay, 10pl Kit. (93-1009E3CP16L)
The PathHunter eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVR1B/BMPR2 Dimerization Assay, 2pl Kit. (93-1072E3CP5M)
The PathHunter ACVR1B/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin A) induced dimerization of ACVR1B/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1B target protein fused to PK alongside BMPR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1B/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress CSF1R Kinase Binding Assay, 10pl Kit. (96-0047E15CP0L)
The InCELL Hunter eXpress CSF1R Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CSF1R via detection of CSF1R levels. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human FGFR3/FGFR3 Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR3/FGFR3 Dimerization Stable Cell Line Assay (U2OS) measures ligand-induced dimerization of FGFR3/FGFR3. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical MitoLite™ Green EX488, 500 test
A mitochondrial dye for live cells; ex/em = 498/520 nm
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0300E2CP0M)
The PathHunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-5 ASY
The Meso Scale Discovery U-PLEX Mouse IL-5 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 5 (IL-5) in serum plasma and cell culture supernatants It uses MSD s SECTOR Imager detecting IL-5 from 0 04 to 1000 pg/mL The kit includes a 96-well plate with capture antibody detection antibody with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours and supports research on mouse models of asthma allergies and other IL-5-related immunological diseases
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.
Genscript Corporation ToxinSensor Single Tests Kit
GenScript ToxinSensor Single Tests Kit with Standard is designed to be a qualitative In Vitro end-point endotoxin test for human and animal parenteral drugs biological products and medical devices Tachypleus Amebocyte Lysate (TAL) as supplied is to be reconstituted with sample or control directly After incubation for 1 hour and in the presence of endotoxin gelation occurs in the absence of endotoxin gelation does not occur GenScript supply a series of ToxinSensorTM Single Tests Kits with Standard with different sensitivity (0 03 EU/ml 0 06 EU/ml 0 125 EU/ml and 0 25 EU/ml)
Eurofins Discoverx cAMP Hunter eXpress ADORA2B CHO-K1 GPCR Assay, 2-Plate Kit. (95-0041E2CP2M)
The cAMP Hunter ADORA2B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. NECA) based activation of ADORA2B GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADORA2B, a naturally Gαs-coupled GPCR. Upon activation of ADORA2B, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0335E2CP2L)
The PathHunter GPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of GPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR1 and EA-tagged β-Arrestin-2. Activation of GPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCNR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0472E2MCP0M)
The PathHunter mCNR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of mCNR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCNR2 and EA-tagged β-Arrestin-2. Activation of mCNR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices FLIPR Calcium 4 Kit, No-Wash, Fluorescence, 10 Plates
Molecular Devices FLIPR Calcium 4 Assay Kit Explorer provides a no-wash, fluorescence-based method for detecting intracellular calcium changes with improved signal-to-noise using a novel masking dye.
- Designed for no-wash fluorescence calcium assays.
- Includes enough reagent for 10 plates in the Explorer configuration.
- Optimized for small calcium responses with reduced background.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Supplied as reagent vials and a dilution/assay buffer to prepare plates.